Coding

Part:BBa_K3268000:Design

Designed by: YI-HSUAN CHAN   Group: iGEM19_NYMU-Taipei   (2019-10-11)


mOR103-15


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 454
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 428
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 834


Design Notes

Cloning it into the vector BBa_J364007 enhances intracellular expression of mouse olfactory receptor mOR103-15 in E. coli.


Source

https://www.addgene.org/15527/

References